ASSESSMENT OF CYTOTOXIC AND APOPTOTIC EFFECTS OF EXTRACTS OF TANACETUM ARGENTEUM SUBSP. ARGENTEUM IN LIVER AND BREAST CANCER CELL LINES
ASSESSMENT OF CYTOTOXIC AND APOPTOTIC EFFECTS OF EXTRACTS OF TANACETUM ARGENTEUM SUBSP. ARGENTEUM IN LIVER AND BREAST CANCER CELL LINES

Summary/Abstract: THE STUDY AIMED TO INVESTIGATE THE ANTICANCER EFFECTS OF HEXANE, CHLOROFORM, METHANOL, AND AQUEOUS METHANOL EXTRACTS OF TANACETUM ARGENTEUM SUBSP. ARGENTEUM (TA) AGAINST BREAST CANCER CELL LINE (MCF-7) AND HUMAN LIVER CANCER CELL LINE (HEPG2) IN VITRO. ANTICANCER, ANTI-INFLAMMATORY, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF TANACETUM SPECIES HAVE BEEN ATTRACTED SCIENTISTS FOR YEARS. CELL VIABILITY WAS DETECTED BY MTT ASSAY AT DIFFERENT CONCENTRATIONS (100, 50, 25, 10, 2.5, AND 1 µG/ML). DRIED AERIAL PARTS OF TA WERE EXTRACTED WITH METHANOL (TAM) AND PARTITIONED BETWEEN WATER AND SUBSEQUENTLY N-HEXANE (TAH) AND CHLOROFORM (TAC).TAC WITH AN IC50VALUE OF 13,66 ΜG/ML EXTRACT ON HEPG2 CELLS AND TAM WITH AN IC50 VALUE OF 48,4 ΜG/ML EXTRACT ON MCF-7 CELLS SHOWED THE MOST POTENT ANTIPROLIFERATIVE ACTIVITY IN A CONCENTRATION-DEPENDENT MANNER AT 24H TIME. THEREFORE, THESE GROUPS ARE USED FOR FURTHER IMMUNOHISTOCHEMICAL ANALYSES. AFP EXPRESSION HAS DETECTED A MEANINGFUL (P< 0,05) INCREASE IN HEPG2 TAC IC50 GROUP COMPARED TO THE CONTROL. THUS, THE RESULTS OF OUR STUDY SUGGEST THAT TAH AND TAC EXTRACTS ON HEPG2 CELLS ALSO TAH AND TAM EXTRACTS ON MCF-7 CELLS MIGHT HAVE HIGH ANTICANCER EFFECTS.

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