Oddziaływanie rosuwastatyny poddanej działaniu czynnika termicznego z wolnymi rodnikami – zastosowanie spektroskopii EPR
Interactions of rosuvastatin effected by thermal factor with free radicals – applications of EPR spectroscpy

Summary/Abstract: The aim of this work was to determine the influence of temperature on interactions of rosuvastatin with free radicals. The drug was heated at temperatures 160°C (120 minutes) and 170°C (60 minutes) in professional sterilizer of Memmert Firm (Niemcy). Temperatures and times of heating of the samples agree with Polish Pharmacopoeia norms for thermal sterilization of drug. The model free radicals as paramagnetic DPPH molecules were used in this study. The quenching of EPR signal of DPPH interacting with drug was analysed. Kinetics of drug – free radicals DPPH interactions was examined. EPR spectra of DPPH were measured by the use of EPR spectrometer of Radiopan Firm (Poznań) and the Rapid Scan Unit system of Jagmar Firm (Kraków). Amplitude (A) and g-factor, were determined. Spectroscopic programms of Jagmar Firm (Kraków) and the programm LabView (National Instruments, USA), were applied. Decrease of amplitude (A) of EPR line of free-radical DPPH caused by interactions with rosuvastatin, for both the drug kept at room temperature and the samples effected by thermal factor. The quenching of EPR signal of free radicals DPPH by the heated rosuvastatin was lower than for the non-heated drug. Rosuvastin heated at temperature 170°C revealed slightly high er interactions with free radicals DPPH compared to this drug heated at temperature 170°C. Usefulness of an X-band (9.3 GHz) EPR spectroscopy to examine interactions of drugs with free radicals was confirmed.

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