WPŁYW JONÓW KADMU NA ALYSSUM MONTANUM W WARUNKACH KULTUR IN VITRO
THE EFFECT OF CADMIUM IONS ON ALYSSUM MONTANUM CULTURED IN VITRO

Summary/Abstract: The aim of this study was to determine an impact of cadmium ions on calamine ecotype of Alyssum montanum, using in vitro culture. The propagation medium was supplemented with different concentrations of cadmium chloride, that is 0.5 μM; 2.5 μM and 5 μM. During the cultivation of microshoots propagation coefficient, leaf blade surface coefficient, fresh, dry weight and numerous biometric features were evaluated. Studied ecotype can be propagated in vitro on modified WPM medium supplemented with cadmium ions, but individual CdCl2 concentrations differentially affected evaluated morphometric traits of studied culture. The higher values of some traits were obtained on media supplemented with cadmium chloride in comparison to control ones. The highest shoot mass (above 11 mg) was obtained on modified WPM supplemented with 0,5 μM CdCl2, whereas the highest number of regenerated shoots were obtained on the same medium supplemented with 5 μM of the same salt.

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