Имобилизиране на фруктозил-трансфераза върху композитни филми от полимлечна киселина, ксантан и хитозан
Immobilization of Functional Transferases on Composite Films of Polylactalic Acid, Xanthan and Chitosan

Summary/Abstract: Fructosyltransferases catalyze the synthesis of Levan type polysaccharides in the presence of sucrose in the reaction medium. In the presence of acceptors (maltose, isomaltose, lactose, fructose) and fructose donor sucrose, they synthesize oligosaccharides with a degree of polymerization DP 3 - 5 monosaccharide residues that have prebiotic potential. In the present study, the fructosyltransferase from Leuconostoc mesenteroides Lm17 strain is immobilized on a multilayer polysaccharide structure composed of chitosan and xanthan. The layers were applied onto pre-loaded corn root cans of polylactic acid. The resulting composite films were investigated for the degree of immobilization of the enzyme and the ability to synthesize fructan on the surface of the film. The activity of immobilized enzyme film was found 0.73 U/mg in the resulting films. In carrying out an enzymatic reaction in the presence of over 10 % sucrose, a hydrolysis and transferase reaction was established by which a polysaccharide was synthesized in both the solution and the membrane. Surface morphology of multilayer films is investigated using the Nanosurf Flex AFM. The values of the average roughness were obtained. It is found that it increases with increasing number of layers, regardless of their sequence.

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