Chemiluminescent and Photometric Determination of the Antioxidant Activity of Cocoon Extracts
Chemiluminescent and Photometric Determination of the Antioxidant Activity of Cocoon Extracts

Summary/Abstract: The extract obtained during the silk production contains sericin, which exhibits good antimicrobial, anticoagulant, antioxidant and other properties that make it interesting for medical applications. In the present paper, we tested the antioxidant activity (AOA) of Bombyx mori cocoon extracts, obtained under soft extraction conditions – with ultrasound and incubation at 60 °C. For this purpose, we used tests for determination of total antioxidant activity (TAOA) – ABTS and DPPH assays, as well as chemiluminescent methods for registration of reactive oxygen species (ROS). The carried-out tests show that the cocoon extracts have AOA determined with the methods for TAOA and at the same time they can influence the quantity of ROS, which makes their activity biologically important. The extracts have stronger AO properties than the ones registered for sericin because of the extraction of additional substances with AO properties most likely with plant origin.

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