Najnowsze techniki biologii molekularnej w profilaktyce przewlekłych zakażeń wirusowych
The latest molecular biology techniques in prevention of chronic viral infections

Summary/Abstract: Progress either in medicine or molecular biology technics induces perspective possibilities of control and complication prevention in case of chronic viral diseases such as viral hepatitis and HIV infection. Accurate control of HBV viremia and seroconvertion level during infection was impossible several years ago. For the last couple of years molecular biology methods for HCV, HBV and HIV infection have been generally accepted and used for diagnosis, even without antibodies detectable in blood. Infection monitoring technics, such as Real-Time PCR, nucleic acid sequencing have been usefull and flow cytofluorometry, have been useful in rapid reacting on changes in the infected patient state. Each of the mentioned technics provides either the infection detection on the level unavailable in the past, or efficient observation and, consequently, proper treatment of the infection. Real-Time PCR allows to define the quantity of viral molecules in the volume of tested blood sample. Sequentioning technic provides viruses type ane virulence definition. The combination of all the other hand the cytofluorometry technic allows to define precisely the quantity of viral proteins presented on the infected cells and detect other blood proteins expression. The combination of all those technics provides accurate definition of virus ammount and efficient treatment

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