Comparative embryonic development of nematodes of the genus Тrichuris (Nematoda, Trichuridae) obtained from sheep (Ovis aries)
Comparative embryonic development of nematodes of the genus Тrichuris (Nematoda, Trichuridae) obtained from sheep (Ovis aries)
Author(s): V. V. Melnychuk, A.V. BerezovskySubject(s): Evaluation research, Environmental interactions
Published by: Дніпропетровський національний університет імені Олеся Гончара
Keywords: trichurosis, Trichuris оvis; Trichuris globulosa; nematode eggs; embryogenesis; in vitro; morphological changes; metric parameters;
Summary/Abstract: Biological specifics of Trichuris оvis Abildgaard, 1795 and T. globulosa Linstow, 1901 parasitizing domestic sheep were analyzed enhancing the species identification of these nematode species. The embryonic development of nematodes was timed, survival of embryonic stages was determined taking into account their morphological and metric specifics in laboratory culture. Trichuris eggs were isolated from gonads of adult female nematodes collected from caeca of dissected sheep. Then the eggs were cultured at optimal temperature to the formation of eggs with mobile larvae. Six morphologically distinct stages of embryogenesis were established in T. оvis and T. globulosa nematodes. The protoplast stage lasted from the 1st to the 12th day in T. оvis and to the 18th day in T. globulosa. Blastomeric formation occurred from the 3rd to the 18th day in T. оvis and from the 3rd to the 21st day in T. globulosa. Bean-shaped embryos formed from the 6th to the 21st day in T. оvis, and from the 9th to the 30th day in T. globulosa
Journal: Biosystems Diversity
- Issue Year: 26/2018
- Issue No: 4
- Page Range: 257-262
- Page Count: 6
- Language: English