Comparative analysis of sample preparation methods for laser scanning cytometry
Comparative analysis of sample preparation methods for laser scanning cytometry
Author(s): Štefan Galbavý, Jozef ŠidloSubject(s): Methodology and research technology, Health and medicine and law, Demography and human biology
Published by: SAMOSATO, s. r. o., Bratislava, Slovensko - MAUREA, s. r. o., Plzeň, Česká republika
Keywords: laser scanning cytometry; cancer; DNA analysis; cell cycle;
Summary/Abstract: Objective: Laser scanning cytometry (LSC) is a technology that combines some features of flow cytometry (FCM), digital microscopy and image analysis. The goal of the work is to verify methodological procedures for processing formalin-fixed and paraffin-embedded tissue, which would be suitable for the analysis of archival material Methods: We processed tissues embedded in paraffin. 10 paraffin blocks with ovarian normal and carcinoma tissue. We prepared the monocellular suspension by enzymatic disintegration with 1 % pepsin. We used the method with heating and without heating. Results and Conclusions: By comparing the results of two methodical procedures without heating and with heating of the tissue section, we found that the methodical procedure without heating is a more suitable method for the preparation of monocellular suspension.
Journal: International Journal of Health, New Technologies and Social Work
- Issue Year: 18/2023
- Issue No: 1
- Page Range: 14-20
- Page Count: 7
- Language: English